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Collagen IV anticorps (AA 1571-1669)

Name: Collagen IV anticorps (AA 1571-1669)
SKU: ABIN707396
Price: 414.62 EUR
Availability: InStock

COL4 Reactivité: Humain, Souris, Rat, Porc ELISA, WB, IHC (fro), IHC (p), FACS, IF (p), IF (cc) Hôte: Lapin Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (ABIN707396) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. (Collagen IV anticorps (AA 1571-1669))

IHC

Formalin-fixed and paraffin embedded human esophageal cancer labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (ABIN707396) at 1:200, followed by conjugation to the secondary antibody and DAB staining (Collagen IV anticorps (AA 1571-1669))

Formalin-fixed and paraffin embedded human esophageal cancer labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (Collagen IV anticorps (AA 1571-1669))

IHC (p)

Downregulation of miR-27a with a miR-27a inhibitor prevented high glucose (HG)-induced mesangial cell (MC) proliferation and the expression of ECM-associated profibrotic genes via upregulation of PPARγ expression. (Collagen IV anticorps (AA 1571-1669))

Inhibition of miR-27a with antagomir-27a reduces the expression of profibrotic genes and ECM proteins in the kidney glomeruli of STZ-induced diabetic rats. (Collagen IV anticorps (AA 1571-1669))

IHC (p)

5 images

(2 références)

N° du produit ABIN707396

Fiche de données

Antigène Voir toutes Collagen IV (COL4) Anticorps

Collagen IV (COL4) (Collagen, Type IV (COL4))
Épitope
16

15

1

1

1

1

1

1

1

1

1

1
AA 1571-1669
Reactivité
90

42

40

22

16

11

5

2
Humain, Souris, Rat, Porc
Hôte
59

27

13
Lapin
Anticorps secondaires appropriés
Clonalité
71

28
Polyclonal
Conjugué
49

10

7

6

3

2

2

2

2

2

2

2

2

2

2

2

1

1
Cet anticorp Collagen IV est non-conjugé
Application
61

49

30

28

27

26

25

25

20

10

9

8

7

3

2

2

1

1

1

1
ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Réactivité croisée

Humain, Souris, Porc, Rat

Homologie

Dog,Cow,Horse,Chicken,Rabbit

Purification

Purified by Protein A.

Immunogène

KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain

Isotype

IgG

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anti-Collagen, Type IV (COL4) antibody (Biotin)
COL4 Reactivité: Humain ELISA Hôte: Chèvre Polyclonal Biotin

Indications d'application

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Sato, Coburn: "Leptospira interrogans causes quantitative and morphological disturbances in adherens junctions and other biological groups of proteins in human endothelial cells." dans: PLoS neglected tropical diseases, Vol. 11, Issue 7, pp. e0005830, (2017) (PubMed).

Wu, Zhang, Ma, Zhang, Qin: "The effect of resveratrol on FoxO1 expression in kidneys of diabetic nephropathy rats." dans: Molecular biology reports, Vol. 39, Issue 9, pp. 9085-93, (2012) (PubMed).
Antigène

Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation

Collagen 4 (COL4 Produits)

Synonymes

anticorps collagen, type IV, anticorps Let-2

Sujet

Synonyms: ICH, HANAC, POREN1, arresten, Collagen alpha-1(IV) chain, COL4A1
Background: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

ID gène

1282

UniProt

P02462