KRT15 anticorps (C-Term)

N° du produit ABIN114888

Cet anticorps anti-KRT15 est un anticorps Souris Monoclonal détectant KRT15 dans IF et IHC (p). Adapté pour Humain, Rat, Souris et Boeuf (Vache).

Aperçu rapide pour KRT15 anticorps (C-Term) (ABIN114888)

Antigène: KRT15 (Keratin 15 (KRT15))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT15 est non-conjugé

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LHK15

Détail du produit anti-KRT15 anticorps

Épitope: C-Term

Specificité: Cytokeratin 15 is a type I cytokeratin without a defined type II partner.
Cellular Localization: Cytoplasmic.

Purification: Purified

Immunogène: A 17-mer synthetic peptide from the C-terminus of human cytokeratin 15

Isotype: IgG2a

Information d'application

Indications d'application: Immunoflourescence. Immunohistochemistry on Paraffin Embedded Sections: 1/25-1/75 dilution in an ABCmethod (30 minutes at room temperature). Requires high temperature antigen unmaskingwith 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur KRT15

Antigène: KRT15 (Keratin 15 (KRT15))

Autre désignation: Cytokeratin 15

Sujet: Cytokeratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. A number of human cytokeratins are expressed during the development of stratified epithelia from one-layered polar epithelia and continue to be expressed in several adult epithelial tissues. Cytokeratin 15 (CK15) is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated. A monoclonal antibody raised against the last 17 amino acids of the CK15 polypeptide show that CK-15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Although CK-15 in normal hair follicles was virtually absent from hair bulbs, it was expressed by a subset of keratinocytes in the outer root sheath. In human conjunctival epithelium, strong expression of CK-15 was observed in basal cells, whereas CK19 was expressed in both basal and suprabasal layers. Although the expression of K15 and K19 differ in humans and mice, specific staining patterns can be used to characterize the epithelial phenotype in normal and diseased ocular surface.Synonyms: CK-15, Cytokeratin-15, K15, KRT15, KRTB, Keratin, Keratin 15, Keratin-15, Type I Cytoskeletal 15

ID gène: 3866

NCBI Accession: NP_002266

UniProt: P19012