ERCC1 anticorps

N° du produit ABIN115126

Détail du produit anti-ERCC1 anticorps

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERCC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificité: This antibody reacts with a 33-36 kDa protein known as ERCC1 polypeptide. Cellular localization: Nuclear. Cross reacts with Rat.

Purification: Protein A Chromatography.

Immunogène: BALB/C mice were injected with full length recombinant human ERCC1 protein.

Clone: 8F1

Isotype: IgG2b

Information d'application

Indications d'application: Immunoprecipitation. Immunohistochemistry on Paraffin Embedded Sections: 1/100-1/200 in an ABC method. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to Immunostaining. Incubation Time: 30 minutes at RT. Recommended Positive Control: Tonsil. Western Blot: 1/100-1/500. use Human lung cell line as Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur ERCC1

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Autre désignation: ERCC1 (ERCC1 Produits)

Synonymes: anticorps COFS4, anticorps RAD10, anticorps UV20, anticorps Ercc-1, anticorps ercc1, anticorps T12H1.18, anticorps T12H1_18, anticorps UV REPAIR DEFICIENT 7, anticorps UVR7, anticorps ERCC excision repair 1, endonuclease non-catalytic subunit, anticorps excision repair cross-complementing rodent repair deficiency, complementation group 1, anticorps DNA excision repair protein ERCC-1, anticorps nucleotide repair protein, anticorps excision repair cross-complementation group 1 L homeolog, anticorps excision repair cross-complementation group 1, anticorps ERCC1, anticorps Ercc1, anticorps CC1G_04546, anticorps CpipJ_CPIJ013903, anticorps PITG_13719, anticorps ercc1, anticorps ercc1.L

Sujet: The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.Synonyms: COFS4, DNA excision repair protein ERCC-1, UV20

ID gène: 2067

NCBI Accession: NP_001974

UniProt: P07992

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response