Podoplanin anticorps

N° du produit ABIN115147

Détail du produit anti-Podoplanin anticorps

Antigène: Podoplanin (PDPN)

Reactivité: Souris

Hôte: Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp Podoplanin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This Syrian Hamster monoclonal antibody will detect the extracellular domain of Podoplanin on the surface of lymphatic endothelial cells and some epithelial cell types.

Aucune reactivité croisée: Humain

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G

Clone: 811

Isotype: IgG

Information d'application

Indications d'application: Immunoprecipitation (1/50). Immunohistochemistry on Frozen and Paraffin Embedded Sections (1/50-1/200). Western blotting (>/= 1/100). The antibody will detect a 36-40 kD band.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: IMMUNOSTAINING ON FORMALIN FIXED PARAFFIN-EMBEDDED SECTIONS1. Deparaffinize through xylenes, ethanol , and hydrate to water. 2. 1% hydrogen peroxide in methanol , 30-40 min. , RT. 3. Wash 2x, dd water, wash in Tris buffer, pH 7. 2, 5 min. each. 4. Proteinase K in 0. 2M Tris, buffer, pH 7. 2, 37°C, 36 µg/ml, 30 min. 5. Wash 3x, 5 min each: Tris buffer, pH 7. 2, dd sterile water, TNT wash buffer. 6. Block in TNB blocking buffer, 30 min. RT. 7. Primary antibody: Hamster anti-mouse Podoplanin antibody 5-10 µg/ml in TNB buffer,overnight, 4°C. 8. Rinse in TNT wash buffer, 3x, 5 min. 9. Incubate with rabbit anti-syrian hamster IgG HRP conjugated, 1: 100 in TNB buffer, 30min. RT. Wash 3x. 10. Biotinylated-tyramide in amplification diluent, 1: 50 , 7 minutes, RT Wash 3x. 11. Incubate in SA-HRP , 1: 100 in TNB, 30 min, RT. Wash 3x. 12. DAB13. Counter stain

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C. Should this product contain a precipitate we recommend microcentrifugation before use.

Références pour anticorps anti-Podoplanin (ABIN115147)

Katayose, Iwata, Oyaizu, Hosono, Yamada, Dang, Hatano, Tanaka, Ohnuma, Morimoto: "The role of Cas-L/NEDD9 as a regulator of collagen-induced arthritis in a murine model." dans: Biochemical and biophysical research communications, Vol. 460, Issue 4, pp. 1069-75, (2015) (PubMed).

Nakao, Zandi, Faez, Kohno, Hafezi-Moghadam: "Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 808-17, (2012) (PubMed).

Noda, Amano, Hata, Kojima, Sawa: "Immunohistochemical examination on the distribution of cells expressed lymphatic endothelial marker podoplanin and LYVE-1 in the mouse tongue tissue." dans: Acta histochemica et cytochemica, Vol. 43, Issue 2, pp. 61-8, (2010) (PubMed).

Uhrin, Zaujec, Breuss, Olcaydu, Chrenek, Stockinger, Fuertbauer, Moser, Haiko, Fässler, Alitalo, Binder, Kerjaschki: "Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation." dans: Blood, Vol. 115, Issue 19, pp. 3997-4005, (2010) (PubMed).

Fonseca, Freund, Bornhäuser, Corbeil: "Polarization and migration of hematopoietic stem and progenitor cells rely on the RhoA/ROCK I pathway and an active reorganization of the microtubule network." dans: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31661-71, (2010) (PubMed).

Gräbner, Lötzer, Döpping, Hildner, Radke, Beer, Spanbroek, Lippert, Reardon, Getz, Fu, Hehlgans, Mebius, van der Wall, Kruspe, Englert, Lovas, Hu, Randolph, Weih, Habenicht: "Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice." dans: The Journal of experimental medicine, Vol. 206, Issue 1, pp. 233-48, (2009) (PubMed).

Davids, Davids, Charlton, Ng, Chong, Laubscher, Dar, Hodge, Soong, Goh: "Response to a novel multitargeted tyrosine kinase inhibitor pazopanib in metastatic Merkel cell carcinoma." dans: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 27, Issue 26, pp. e97-100, (2009) (PubMed).

Gohda, Kunisawa, Miura, Kagiyama, Kurashima, Higuchi, Ishikawa, Ogahara, Kiyono: "Sphingosine 1-phosphate regulates the egress of IgA plasmablasts from Peyer's patches for intestinal IgA responses." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 8, pp. 5335-43, (2008) (PubMed).

Détails sur Podoplanin

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin (PDPN Produits)

Synonymes: anticorps PDPN, anticorps AGGRUS, anticorps GP36, anticorps GP40, anticorps Gp38, anticorps HT1A-1, anticorps OTS8, anticorps PA2.26, anticorps T1A, anticorps T1A-2, anticorps OTS-8, anticorps RANDAM-2, anticorps T1-alpha, anticorps T1a, anticorps T1alpha, anticorps E11, anticorps RTI40, anticorps podoplanin, anticorps PDPN, anticorps Pdpn

Sujet: Podoplanin is a well-recognized lymphatic endothelium marker, which can be used to reliably distinguish lymphatic vessels from blood vessels. Podoplanin is a Mr 38,000 membrane mucoprotein that was originally detected on the surface of rat glomerular epithelial cells (podocytes) and was found to be linked to flattening of foot processes that occurs in glomerular diseases. Podoplanin shows features of a membrane mucoprotein with several conserved O-glycosylation sites. Currently, it is of unknown biological function. Because heavily O-glycosylated mucoproteins were identified recently as counterreceptors for selectins that mediate adhesion of inflammatory cells, it is possible that podoplanin plays a similar role in lymphatic endothelia.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

ID gène: 14726

NCBI Accession: NP_034459

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport