MSH6 anticorps

N° du produit ABIN115272

Cet anticorps anti-MSH6 est un anticorps Souris Monoclonal détectant MSH6 dans WB, IHC (p), IP et IHC (fro). Adapté pour Humain, Souris, Rat et Chien.

Aperçu rapide pour MSH6 anticorps (ABIN115272)

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 44

Détail du produit anti-MSH6 anticorps

Specificité: MSH6 is a heterodimer of MSH2 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop.
Cellular Localization: Nuclear.

Purification: Affinity Chromatography.

Immunogène: Synthetic human MSH6 peptide

Isotype: IgG1

Information d'application

Indications d'application: Immunoprecipitation. Western Blot: 1/100-1/500. Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod (30 to 60 minutes at room temperature (requires high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining). Immunohistochemistry on Frozen Sections. Recommended Positive Control: Tonsil, colon carcinoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MSH6

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Autre désignation: MSH6

Sujet: An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time.Synonyms: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, MutS-alpha 160 kDa subunit, p160

ID gène: 2956

NCBI Accession: NP_000170

UniProt: P52701

Pathways: Réparation de l'ADN, Chromatin Binding, Production of Molecular Mediator of Immune Response