Western blot (0.5-1 μg/mL): The antibody was tested using several His-tagged proteinsexpressed in insect cells and E. coli. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of > 0.5 mg/mL.
Buffer
PBS, pH 7.4, without preservatives.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
The lyophilized IgG is stable at 2-8 °C for one month from despatch and for one year when kept at -20 °C. The reconstituted antibody can be stored at 2-8 °C for one month or at -20 °C for one year.
Date de péremption
12 months
Antigène
His Tag
Autre désignation
6xHistidine Epitope Tag
Classe de substances
Tag
Sujet
In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called