IL-4 anticorps

N° du produit ABIN115763

Détail du produit anti-IL-4 anticorps

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-4 est non-conjugé

Application: Functional Studies (Func), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Reacts with Interleukin 4.

Purification: Protein A chromatography

Immunogène: Highly pure (>98%) recombinant human IL-4

Isotype: IgG1

Information d'application

Indications d'application: ELISA: A 1: 10,000 dilution of a 1 mg/mL antibody solution will give satisfactory results in anELISA using a rabbit anti-mouse second antibody conjugated to alkaline phosphatase. Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-4 (0.5 ng/mL), a concentration of 3.0-4.5 μg/mL is required. Western blot: Use at a concentration of 0.20-0.40 μg/mL. Used in conjunction withcompatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur IL-4

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: Interleukin-4 / IL4 (IL4 Produits)

Synonymes: anticorps IL4, anticorps IL-4, anticorps BCGF-1, anticorps BCGF1, anticorps BSF-1, anticorps BSF1, anticorps Il-4, anticorps Il4e12, anticorps IL2, anticorps interleukin 4, anticorps IL4, anticorps Il4

Sujet: IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

ID gène: 3565

NCBI Accession: NP_000580

UniProt: P05112

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation