GM-CSF anticorps

N° du produit ABIN115853

L’anticorps anti-GM-CSF Polyclonal Lapin est utilisé pour la détection de GM-CSF dans des échantillons de Souris. Il a été validé pour WB, EIA et Func.

Aperçu rapide pour GM-CSF anticorps (ABIN115853)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GM-CSF est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-GM-CSF anticorps

Specificité: This antibody reacts with Mouse GM-CSF.

Purification: Affinity Chromatography employing immobilized mGM-CSF matrix.

Immunogène: E.coli derived Highly pure (>98%) recombinant Murine GM-CSF Remarks: Mol. Weight: ~150 kDa

Information d'application

Indications d'application: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/mL), a oncentration of 0.012-0.02 μg/mL of this antibody is required. ELISAIndirect: To detect Mouse GM-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Mouse GM-CSF. Sandwich: To detect mGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine GM-CSF (PP003B1 or PP003B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmGM-CSF. Western Blot: To detect Mouse GM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur GM-CSF

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GM-CSF

Sujet: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

ID gène: 12981

NCBI Accession: NP_034099

UniProt: P01587

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin