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IL-9 anticorps

Cet anticorps anti-IL-9 est un anticorps Lapin Polyclonal détectant IL-9 dans WB, EIA et Func. Adapté pour Souris.
N° du produit ABIN115893

Aperçu rapide pour IL-9 anticorps (ABIN115893)

Antigène

Voir toutes IL-9 (IL9) Anticorps
IL-9 (IL9) (Interleukin 9 (IL9))

Reactivité

  • 43
  • 41
  • 8
  • 2
  • 1
  • 1
  • 1
  • 1
Souris

Hôte

  • 55
  • 18
  • 9
  • 3
  • 2
  • 2
Lapin

Clonalité

  • 52
  • 34
  • 3
Polyclonal

Conjugué

  • 54
  • 9
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp IL-9 est non-conjugé

Application

  • 58
  • 31
  • 25
  • 23
  • 14
  • 13
  • 13
  • 12
  • 7
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
  • Specificité

    Recognises Murine IL-9

    Purification

    Affinity chromatography.

    Immunogène

    Highly pure (>98%) recombinant mIL-9
  • Indications d'application

    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-9 (0.30 ng/mL),a concentration of 0.006-0.012 μg/mL of this antibody is required. ELISA: To detect mIL-9 by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mIL-9. Western Blot: To detect mIL-9 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    Buffer

    PBS, pH 7.2 without preservatives.

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
  • Antigène

    IL-9 (IL9) (Interleukin 9 (IL9))

    Autre désignation

    Interleukin-9 / IL9

    Sujet

    Interleukin 9 (IL9) is a cytokine preferentially produced by T helper type 2 lymphocytes and active on various cell types such as T and B lymphocytes, mast cells and haemopoietic progenitors, but no effect of this cytokine has been reported so far on mononuclear phagocytes. IL9 stimulates cell proliferation and prevents apoptosis. The IL9 receptor (IL 9R) belongs to the haemopoietic receptor superfamily and its signal transduction involves mainly the (JAK/STAT) pathway. Some studies have implicated IL9 as an important cytokine in a number of Th2 cytokine mediated pathologies, in particular asthma.Synonyms: IL-9, P40 cytokine, T-cell growth factor P40

    ID gène

    16198

    NCBI Accession

    NP_032399

    UniProt

    P15247

    Pathways

    Signalistation JAK/STAT
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