BDNF anticorps (Biotin)

N° du produit ABIN115983

Détail du produit anti-BDNF anticorps (Biotin)

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BDNF est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: Recognizes Human BDNF.

Réactivité croisée (Details): Species reactivity (tested):Human

Purification: Affinity chromatography

Immunogène: Highly purified (>98%) recombinant Human BDNF

Information d'application

Indications d'application: ELISA: Direct: To detect Human BDNF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human BDNF. Sandwich: To detect Human BDNF by Sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Anti-Human BDNF (PP1000P1 or PP1000P1) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BDNF. Western blot: To detect Human BDNF by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Références pour anticorps anti-BDNF (Biotin) (ABIN115983)

Hartog, Dittrich, Pieneman, Jansen, Frankl-Vilches, Lessmann, Lilliehook, Goldman, Gahr: "Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 49, pp. 15511-9, (2009) (PubMed).

Détails sur BDNF

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Autre désignation: BDNF (BDNF Produits)

Synonymes: anticorps ANON2, anticorps BULN2, anticorps bdnf-A, anticorps BDNF, anticorps bdnf, anticorps brain derived neurotrophic factor, anticorps brain-derived neurotrophic factor, anticorps brain-derived neurotrophic factor L homeolog, anticorps brain-derived neurotrophic factor S homeolog, anticorps BDNF, anticorps Bdnf, anticorps bdnf, anticorps bdnf.L, anticorps bdnf.S

Sujet: Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55 % sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.Synonyms: Abrineurin, Brain-derived neurotrophic factor

ID gène: 627

NCBI Accession: NP_001137277

UniProt: P23560

Pathways: Signalisation RTK, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity