Cardiotrophin 1 anticorps

N° du produit ABIN115989

Cet anticorps anti-Cardiotrophin 1 est un anticorps Lapin Polyclonal détectant Cardiotrophin 1 dans WB, EIA et Func. Adapté pour Humain.

Aperçu rapide pour Cardiotrophin 1 anticorps (ABIN115989)

Antigène: Cardiotrophin 1 (CTF1)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cardiotrophin 1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-Cardiotrophin 1 anticorps

Specificité: This antibody reacts with Human Cardiotrophin-1

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hCardiotrophin-1.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhCardiotrophin-1 (3 ng/mL), a concentration of 0.04-0.09 μg/mL of this antibody isrequired. ELISA: To detect hCardiotrophin-1 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hCardiotrophin-1. Western Blot: To detect hCardiotrophin-1 by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCardiotrophin-1 is 1.5-3.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur Cardiotrophin 1

Antigène: Cardiotrophin 1 (CTF1)

Autre désignation: Cardiotrophin-1

Sujet: Cardiotrophin-1 (CT-1), a cardiac hypertrophic factor, is a 21.5 kDa protein member of the IL-6 cytokine family. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. The protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer. In addition, CT-1 activates phosphatidylinositol 3-kinase (PI-3 kinase) in cardiac myocytes and enhances transcription factor NF kappa B DNA -binding activities. CT-1 is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine.Synonyms: CT-1, CTF1

ID gène: 1489

NCBI Accession: NP_001136016

UniProt: Q16619

Pathways: Signalistation JAK/STAT