FLT3LG anticorps

N° du produit ABIN116013

Détail du produit anti-FLT3LG anticorps

Antigène: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FLT3LG est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Specificité: Human flt3-Ligand.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hflt3-Ligand

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFlt-3 Ligand (15 ng/mL), a concentration of 0.25-0.75 μg/mL of this antibody is required. ELISA: (Indirect): To detect hFlt-3 Ligand by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFlt-3 Ligand. (Sandwich): To detect hFlt-3 Ligand by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human Flt-3 Ligand (PP1008B1,PP1008B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hFlt-3 Ligand. Western Blot: To detect hFlt-3 Ligand by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFlt-3 Ligand is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Centrifuge vial prior to opening!

Stock: 4 °C

Stockage commentaire: Store the antibody at 2-8 °C. DO NOT FREEZE! Avoid prolonged exposure to light.

Détails sur FLT3LG

Antigène: FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))

Autre désignation: Flt3 Ligand (FLT3LG Produits)

Synonymes: anticorps Flt3lg, anticorps Ly72L, anticorps FL, anticorps FLT3L, anticorps Vegfr3, anticorps fms related tyrosine kinase 3 ligand, anticorps FMS-like tyrosine kinase 3 ligand, anticorps fms-related tyrosine kinase 4, anticorps fms-related tyrosine kinase 3 ligand, anticorps FLT3LG, anticorps Flt3l, anticorps Flt4, anticorps Flt3lg

Sujet: Flt3 ligand is a ligand for the FLT3 tyrosine kinase receptor and belongs to a small group of growth factors that regulate proliferation of early hematopoietic cells. Multiple isoforms of Flt3 ligand have been identified. The predominant form is the transmembrane form, which is biologically active on the cell surface. When proteolytically cleaved the transmembrane isoform generates a soluble form, which is also biologically active. Flt3 ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3 ligand alone cannot stimulate proliferation, but synergizes well with other CSFs and interleukins to induce growth and differentiation.Synonyms: FLT3LG, Flt3L, SL cytokine, fms-related tyrosine kinase 3 ligand

ID gène: 2323

NCBI Accession: NP_001191431

UniProt: P49771

Pathways: Signalisation RTK