IL1A anticorps (Biotin)

N° du produit ABIN116061

Cet anticorps anti-IL1A est un anticorps Lapin Polyclonal détectant IL1A dans WB et EIA. Adapté pour Humain.

Aperçu rapide pour IL1A anticorps (Biotin) (ABIN116061)

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL1A est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL1A anticorps (Biotin)

Specificité: This antibody recognizes Human Interleukin-1 alpha (IL-1 alpha).

Purification: Affinity Chromatography.

Immunogène: Highly pure (>98%) recombinant hIL-1 alpha (human IL-1 alpha).

Information d'application

Indications d'application: ELISA: To detect hIL-1 alpha by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthIL-1 alpha. Western Blot: To detect hIL-1 alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-1 alpha is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Détails sur IL1A

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: Interleukin-1 alpha / IL-1A

Sujet: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

ID gène: 3552

NCBI Accession: NP_000566

UniProt: P01583

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints