IL1A anticorps

N° du produit ABIN116063

Détail du produit anti-IL1A anticorps

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL1A est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: This antibody reacts with Human Interleukin-1 alpha.

Purification: Affinity chromatography.

Immunogène: Highly purified (>98%) recombinant Interleukin 1 alpha.

Information d'application

Indications d'application: Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect hIL-1alpha by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hIL-1 alpha. Western Blot: Use a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant hIL-1alpha is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1alpha (0.1 ng/mL), a concentration of 0.01-0.01 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 2-8 °C for one month, or (in aliquots) at -20 °C for longer.

Détails sur IL1A

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: Interleukin-1 alpha / IL-1A (IL1A Produits)

Synonymes: anticorps IL-1A, anticorps IL1, anticorps IL1-ALPHA, anticorps IL1F1, anticorps IL-6, anticorps Il-1a, anticorps IL-1 alpha, anticorps IL-1alpha, anticorps L1A, anticorps interleukin 1 alpha, anticorps interleukin 6, anticorps IL1A, anticorps IL6, anticorps Il1a

Sujet: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

ID gène: 3552

NCBI Accession: NP_000566

UniProt: P01583

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints