IL-5 anticorps
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- Antigène Voir toutes IL-5 (IL5) Anticorps
- IL-5 (IL5) (Interleukin 5 (IL5))
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp IL-5 est non-conjugé
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
- Specificité
- This antibody reacts with Human Interleukin-5. Other species not tested.
- Purification
- Affinity Chromatography.
- Immunogène
- Highly pure (>98%) recombinant Human IL-5.
- Top Product
- Discover our top product IL5 Anticorps primaire
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- Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5(0.45 ng/mL), a concentration of 0.05-0.09 μg/mL of this antibody is required. ELISA: To detect hIL-5 by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Antigène
- IL-5 (IL5) (Interleukin 5 (IL5))
- Autre désignation
- Interleukin-5 / IL5 (IL5 Produits)
- Synonymes
- anticorps EDF, anticorps IL-5, anticorps TRF, anticorps Il-5, anticorps IL5, anticorps interleukin 5, anticorps IL5, anticorps Il5
- Sujet
- Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
- ID gène
- 3567
- NCBI Accession
- NP_000870
- UniProt
- P05113
- Pathways
- Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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