IL-7 anticorps (Biotin)

N° du produit ABIN116086

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-7 dans WB et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour IL-7 anticorps (Biotin) (ABIN116086)

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-7 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL-7 anticorps (Biotin)

Specificité: Human IL-7

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hIL-7 (human IL-7).

Information d'application

Indications d'application: ELISA: To detect hIL-7 by direct ELISA (using 100 ml/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-7. Western Blot: To detect hIL-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 4-8 °C for one month or at -20 °C for longer.

Détails sur IL-7

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Autre désignation: Interleukin-7 / IL7

Sujet: Interleukin 7 (IL7) is a lymphoid cell growth factor that affects pre-B, pro-B, and early T cells. IL7 was previously known as pre-B cell growth factor and lymphopoietin 1. IL7 supports the growth of early B cells from long-term lymphoid bone marrow cultures. It is mitogenic to thymocytes and enhances the response of cells to other stimuli such as polyhydroxyalkanoate (PHA) and concanavalin A (ConA). IL7 stimulates the proliferation of CD4+/CD8+ cells. The proliferative response of thymocytes to IL7 is not affected by antibodies to the T cell growth factors such as IL2, IL4 and IL6, suggesting that IL7 is capable of stimulating T cell proliferation through a pathway independent of the known T cell growth factors. Mature T cells respond to IL7 and Con A, but not to IL7 alone. In myeloid lineage cells, IL7 upregulates the production of pro-inflammatory cytokines and stimulates the tumoricidal activity of monocytes/macrophages. IL7 is expressed by adherent stromal cells from various tissues.Synonyms: IL-7

ID gène: 3574

NCBI Accession: NP_000871

UniProt: P13232

Pathways: Signalistation JAK/STAT