M-CSF/CSF1 anticorps

N° du produit ABIN116156

Détail du produit anti-M-CSF/CSF1 anticorps

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp M-CSF/CSF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: Human Macrophage Colony Stimulating Factor (M-CSF)

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hM-CSF (human M-CSF).

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhM-CSF (7.0 ng/mL), a concentration of 0.02-0.03 μg/mL of this antibody is required. ELISA: (Indirect): To detect hM-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hM-CSF. (Sandwich): To detect hM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human M-CSF (PP1048B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Détails sur M-CSF/CSF1

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Autre désignation: Macrophage Colony Stimulating Factor (M-CSF) (CSF1 Produits)

Synonymes: anticorps CSF-1, anticorps MCSF, anticorps C87615, anticorps Csfm, anticorps op, anticorps csf1-1, anticorps zgc:172186, anticorps CSF1, anticorps csf1-2, anticorps zgc:158436, anticorps colony stimulating factor 1, anticorps colony stimulating factor 1 (macrophage), anticorps colony stimulating factor 1a (macrophage), anticorps macrophage colony-stimulating factor 1, anticorps macrophage colony stimulating factor, anticorps colony stimulating factor 1b (macrophage), anticorps CSF1, anticorps Csf1, anticorps csf1a, anticorps LOC396599, anticorps LOC100860895, anticorps csf1b

Sujet: Synonyms: CSF-1, CSF1, MCSF, Macrophage colony-stimulating factor 1

ID gène: 1435

NCBI Accession: NP_000748

UniProt: P09603

Pathways: Signalisation RTK