Nerve Growth Factor anticorps (Biotin)

N° du produit ABIN116189

L’anticorps Lapin Polyclonal anti-Nerve Growth Factor a été validé pour WB et EIA. Il convient pour détecter Nerve Growth Factor dans des échantillons de Humain.

Aperçu rapide pour Nerve Growth Factor anticorps (Biotin) (ABIN116189)

Antigène: Nerve Growth Factor (NGF)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nerve Growth Factor est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Nerve Growth Factor anticorps (Biotin)

Specificité: Specific for Human beta NGF (Nerve Growth Factor). Other species not tested.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hbeta-NGF (Anti-human beta-Nerve Growth Factor).

Information d'application

Indications d'application: ELISA: To detect hbeta-NGF by direct ELISA (using 100 ml/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthbeta-NGF. Western Blot: To detect hbeta-NGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hbeta-NGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur Nerve Growth Factor

Antigène: Nerve Growth Factor (NGF)

Autre désignation: NGF

Sujet: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

ID gène: 4803

NCBI Accession: NP_002497

UniProt: P01138

Pathways: Regulation of Cell Size