CXCL12 anticorps

N° du produit ABIN116233

Détail du produit anti-CXCL12 anticorps

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CXCL12 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: Reacts with Stromal Cell Derived Factor-1 beta (SDF-1 beta).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogène: E. Coli derived Recombinant recombinant Human SDF-1 beta

Isotype: IgG

Information d'application

Indications d'application: Indirect ELISA: To detect Human SDF-1 beta by Indirect ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with compatible secondary reagents, allows thedetection of 0.2-0.4 ng/well of recombinant Human SDF-1 beta. Sandwich ELISA: To detect Human SDF-1 beta by Sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Thisantigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SDF-1 beta(PP1068B1 or PP1068B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF-1 beta. Western Blot: To detect Human SDF-1 beta by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hSDF-1 bet is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening! Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CXCL12

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Autre désignation: CXCL12 / SDF1 (CXCL12 Produits)

Synonymes: anticorps IRH, anticorps PBSF, anticorps SCYB12, anticorps SDF1, anticorps TLSF, anticorps TPAR1, anticorps CINC-1, anticorps Gro1, anticorps Pbsf, anticorps Scyb12, anticorps Sdf1, anticorps Tlsf, anticorps Tpar1, anticorps Fsp, anticorps KC, anticorps Mgsa, anticorps N51, anticorps Scyb1, anticorps gro, anticorps sdf-1, anticorps sdf1, anticorps xSDF-1, anticorps SDF-1, anticorps cxcl12, anticorps sdf-1a, anticorps sdf1a, anticorps wu:fa55e10, anticorps wu:fc16h12, anticorps wu:fj84c02, anticorps C-X-C motif chemokine ligand 12, anticorps C-X-C motif chemokine ligand 1, anticorps chemokine (C-X-C motif) ligand 12, anticorps chemokine (C-X-C motif) ligand 1, anticorps C-X-C motif chemokine ligand 12 L homeolog, anticorps chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1), anticorps CXCL12, anticorps cxcl12, anticorps Cxcl1, anticorps Cxcl12, anticorps cxcl12.L, anticorps cxcl12a

Sujet: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Poids moléculaire: approx. 150 kDa

ID gène: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling