Thrombopoietin anticorps

N° du produit ABIN116255

Détail du produit anti-Thrombopoietin anticorps

Antigène: Thrombopoietin (THPO)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Thrombopoietin est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: This antibody reacts with Thrombopoietin.

Purification: Affinity chromatography.

Immunogène: Highly purified recombinant human TPO.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTPO (5.00 ng/mL), a concentration of 4.2-6.3 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTPO by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTPO. (Sandwich): To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur Thrombopoietin

Antigène: Thrombopoietin (THPO)

Autre désignation: Thrombopoietin (THPO Produits)

Synonymes: anticorps MGDF, anticorps MKCSF, anticorps ML, anticorps MPLLG, anticorps THCYT1, anticorps TPO, anticorps Mgdf, anticorps Ml, anticorps Mpllg, anticorps Tpo, anticorps Tpo1, anticorps Tpo2, anticorps Tpo3, anticorps Tpo4, anticorps tpo, anticorps LOC100231762, anticorps thrombopoietin, anticorps THPO, anticorps Thpo, anticorps thpo

Sujet: Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO

ID gène: 7066

NCBI Accession: NP_000451

UniProt: P40225

Pathways: Signalistation JAK/STAT, Hormone Activity