GM-CSF anticorps (Biotin)
Aperçu rapide pour GM-CSF anticorps (Biotin) (ABIN116303)
Antigène
Voir toutes GM-CSF (CSF2) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Reacts with GM-CSF
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Purification
- Affinity chromatography.
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Immunogène
- Highly pure (>98%) recombinant hGM-CSF (human GM-CSF).
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Indications d'application
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ELISA: To detect hGM-CSF by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthGM-CSF. Western Blot: To detect hGM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGM-CSF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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Autre désignation
- GM-CSF
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Sujet
- The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim
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ID gène
- 1437
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NCBI Accession
- NP_000749
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UniProt
- P04141
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Pathways
- Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin
Antigène
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