GM-CSF anticorps

N° du produit ABIN116305

Cet anticorps Lapin Polyclonal détecte spécifiquement GM-CSF dans WB, EIA, IHC (p) et Func. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour GM-CSF anticorps (ABIN116305)

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GM-CSF est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Détail du produit anti-GM-CSF anticorps

Specificité: This antibody recognizes Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF). Other species not tested.

Purification: Affinity Chromatography employing immobilized hGM-CSF matrix

Immunogène: Highly pure (>98%) E.coli derived recombinant Human GM-CSF (Human Granulocyte Macrophage Colony Stimulating Factor)

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGMCSF (1.0 ng/mL), a concentration of 0.30-0.50 μg/mL of this antibody is required. ELISA: To detect hGM CSF by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hGM CSF. Western Blot: To detect hGM CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGM CSF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur GM-CSF

Antigène: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Autre désignation: GM-CSF

Sujet: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

ID gène: 1437

NCBI Accession: NP_000749

UniProt: P04141

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin