IL12A anticorps

N° du produit ABIN116309

L’anticorps Chèvre Polyclonal anti-IL12A a été validé pour WB, EIA et Func. Il convient pour détecter IL12A dans des échantillons de Humain.

Aperçu rapide pour IL12A anticorps (ABIN116309)

Antigène: IL12A (Interleukin 12 alpha (IL12A))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IL12A est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-IL12A anticorps

Specificité: Interleukin-12 (IL-12)

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hIL-12 (human Interleukin-12)

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-12 (0.3 ng/mL), a concentration of 0.012 - 0.02 μg/mL of this antibody is required. ELISA: To detect hIL-12 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hIL-12. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur IL12A

Antigène: IL12A (Interleukin 12 alpha (IL12A))

Autre désignation: Interleukin-12 alpha / IL12A

Sujet: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

ID gène: 3592

NCBI Accession: NP_000873

UniProt: P29459

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation