TWEAK anticorps

N° du produit ABIN116357

L’anticorps anti-TWEAK Polyclonal Chèvre est utilisé pour la détection de TWEAK dans des échantillons de Humain. Il a été validé pour WB, Func et EIA.

Aperçu rapide pour TWEAK anticorps (ABIN116357)

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp TWEAK est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Détail du produit anti-TWEAK anticorps

Specificité: Human TWEAK

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hTWEAK.

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTWEAK (200 ng/mL), a concentration of 0.1 - 0.2 μg/mL of this antibody is required. ELISA: To detect hTWEAK by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTWEAK is 1.5-3.0 ng/lane, under either reducing or ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur TWEAK

Antigène: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Autre désignation: TWEAK / TNFSF12

Sujet: Synonyms: APO3 ligand, APO3L, DR3LG, TNF-related weak inducer of apoptosis, Tumor necrosis factor ligand superfamily member 12

ID gène: 8742

NCBI Accession: NP_003800

UniProt: O43508

Pathways: Apoptose