CXCL12 anticorps

N° du produit ABIN116457

Cet anticorps anti-CXCL12 est un anticorps Chèvre Polyclonal détectant CXCL12 dans WB, EIA et Neut. Adapté pour Souris.

Aperçu rapide pour CXCL12 anticorps (ABIN116457)

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CXCL12 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)

Détail du produit anti-CXCL12 anticorps

Specificité: Reacts with Mouse Stromal Cell Derived Factor-1 alpha (SDF-1 alpha). Other species not tested.

Purification: Affinity Chromatography employing immobilized Murine SDF-1α matrix

Immunogène: Highly pure (>98%) E.coli derived recombinant Mouse SDF-1 alpha (Cat.-No PA152) Remarks: Molecular Weight: ~150 kDa

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofMurine SDF-1 alpha (100 ng/mL), a concentration of 2.5-3.5 μg/mL of this antibody isrequired. Sandwich ELISA: To detect Mouse SDF-1 alpha by direct ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with compatible secondary reagents, allows thedetection of of 0.2-0.4 ng/well of recombinant Mouse SDF-1 alpha. Western Blot: To detect Mouse SDF-1 alpha by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant mSDF-1 alpha is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CXCL12

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Autre désignation: CXCL12 / SDF1

Sujet: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

ID gène: 20315

NCBI Accession: NP_001012495

UniProt: P40224

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling