BMP2 anticorps

N° du produit ABIN116466

Détail du produit anti-BMP2 anticorps

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BMP2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: The antibody recognizes Human BMP2. Other species not tested.

Purification: Affinity Chromatography employing immobilized Human BMP-2 matrix

Immunogène: Highly pure (>98%) E.coli derived recombinant Human BMP-2.

Information d'application

Indications d'application: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200 ng/mL), a concentration of 1.3-1.9 μg/mL of this antibody is required. ELISAIndirect: To detect hBMP-2 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBMP-2. Sandwich: To detect hBMP-2 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with a biotinylated Anti-Human BMP-2 (PP1219B) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-2. Western BlotTo detect hBMP-2 by Western Blot analysis this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBMP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge the vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur BMP2

Antigène: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Autre désignation: BMP2 / BMP2A (BMP2 Produits)

Synonymes: anticorps BDA2, anticorps BMP2A, anticorps AI467020, anticorps Bmp2a, anticorps BMP-2, anticorps xBMP-2, anticorps xbmp2, anticorps BMP2, anticorps bmp2a, anticorps bmp2, anticorps wu:fc59d09, anticorps bone morphogenetic protein 2, anticorps bone morphogenetic protein 2 L homeolog, anticorps Bone morphogenetic protein 2, anticorps bone morphogenetic protein 2a, anticorps BMP2, anticorps Bmp2, anticorps bmp2.L, anticorps bmp2, anticorps bmp2a

Sujet: BMP2 belongs to the transforming growth factor beta (TGFB) superfamily of secreted growth factors. It is a disulfide linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.Synonyms: BMP-2, BMP-2A, Bone morphogenetic protein 2

ID gène: 650

NCBI Accession: NP_001191

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation