Cyclin T1 anticorps (Internal Region)

N° du produit ABIN116513

Détail du produit anti-Cyclin T1 anticorps

Antigène: Cyclin T1 (CCNT1)

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin T1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Antiserum will specifically react with a 85 kDa cyclin T1 protein. No reaction was observed against other related cyclins.

Purification: Delipidation and defibrination

Immunogène: Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Information d'application

Indications d'application: Suitable for ELISA, immunoprecipitation, immunohistochemistry, immunoblotting andother immunological methods requiring high titer and specificity. Recommended dilutions: ELISA 1: 1,000 - 1: 5,000Western blot 1: 500 - 1: 2,000Immunohistochemistry 1: 200 - 1: 1,000 (use paraffin embedded tissue).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 75 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.01 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C to -80 °C. Avoid multiple freeze/thaw cycles.

Détails sur Cyclin T1

Antigène: Cyclin T1 (CCNT1)

Autre désignation: Cyclin T1 (CCNT1 Produits)

Synonymes: anticorps CG6292, anticorps Dmcyclin T, anticorps Dmel\\CG6292, anticorps ORE-14, anticorps P-TEFb, anticorps anon-74EFc, anticorps dT, anticorps p124, anticorps Cyclin-T1, anticorps ccnt, anticorps cyct1, anticorps CCNT, anticorps CYCT1, anticorps HIVE1, anticorps 2810478G24Rik, anticorps AI115585, anticorps CycT1, anticorps fi75b02, anticorps si:dkey-18f23.10, anticorps wu:fi75b02, anticorps Cyclin T, anticorps cyclin T1, anticorps cyclin T1 L homeolog, anticorps CycT, anticorps CCNT1, anticorps ccnt1, anticorps Ccnt1, anticorps ccnt1.L

Sujet: Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.Synonyms: CCNT1, CycT1, Cyclin-T, Cyclin-T1

ID gène: 904

NCBI Accession: NP_001231

UniProt: O60563