This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with ApoLipoprotein A-II and has negligible cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible.
Purification
Immunoaffinity Chromatography.
Immunogène
ApoLipoprotein Type A-II was isolated from Human plasma by Density Gradient Centrifugation followed by HPLC purification.
This Apo AII antibody R1031P has been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and Western blotting forhighly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Western blot: 1/5,000-1/10,000. Immunohistochemistry: 1: /50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01 % Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.Synonyms: APOA2, Apo-AII, ApoA-II, Apolipoprotein A-II, Apolipoprotein A2