APOE anticorps

N° du produit ABIN116547

Cet anticorps anti-APOE Polyclonal Chèvre (ABIN116547) détecte spécifiquement APOE dans WB, IP, IHC (p), IHC (fro) et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour APOE anticorps (ABIN116547)

Antigène: APOE (Apolipoprotein E (APOE))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APOE est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-APOE anticorps

Specificité: This product has been prepared by Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other Apolipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human Apolipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J Apolipoproteins. Specific cross reaction of anti-Apolipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-Apolipoprotein antibodies with other human serum proteins is negligible.

Purification: Immunoaffinity Chromatography

Immunogène: Apolipoprotein Type E was isolated from Human plasma by density gradient centrifugation followed by HPLC purification.

Information d'application

Indications d'application: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitationof antigen in serum using a standard curve, for immunoprecipitation and for westernblotting for highly sensitive qualitative analysis. Recommended dilutions: ELISA: 1/4,000-1/8,000Western blot: 1/500-1/2,000Immunohistochemistry: 1/50-1/200
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, containing 0.01 % Sodium Azide as preservtive

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquts) at -20 °C for longer.

Détails sur APOE

Antigène: APOE (Apolipoprotein E (APOE))

Autre désignation: Apolipoprotein E (Apo E)

Sujet: Apolipoprotein E is essential for the normal catabolism of triglyceride rich lipoprotein constituents. The apolipoprotein E gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in Apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Mutations in the APOE gene confer susceptibility to Alzheimer's disease by affecting amyloid-beta deposition.Synonyms: Apo-E, ApoE

ID gène: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism