ADA anticorps
Aperçu rapide pour ADA anticorps (ABIN116575)
Antigène
Voir toutes ADA AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species are not specifically determined, but are very probable due to high sequence homologie (human >90 % , mouse/rat 85 %).
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Purification
- Prepared from monospecific antiserum by a delipidation and defibrination.
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Immunogène
- Adenosine Deaminase from Calf Spleen.
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Indications d'application
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 0.3 μg of AdenosineDeaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (R1364HRP) and DAB as a substrate at room temperature. Aworking dilution of 1: 5,000 to 1: 10,000 of the reconstitution concentration is suggested forthis product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Restore with 2.0 mL of deionized water (or equivalent).
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Concentration
- 90.0 mg/mL (by Refractometry)
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- ADA (Adenosine Deaminase (ADA))
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Sujet
- Synonyms: Adenosine aminohydrolase
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ID gène
- 280712
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NCBI Accession
- NP_776312
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UniProt
- P56658
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Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
Antigène
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