Alkaline Phosphatase anticorps

N° du produit ABIN116596

Détail du produit anti-Alkaline Phosphatase anticorps

Antigène: Alkaline Phosphatase (ALP)

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Alkaline Phosphatase est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificité: This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.

Purification: Delipidation and Defibrination

Immunogène: Alkaline Phosphatase [E.coli].

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 μg of Alkaline Phosphatase [E. coli] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000Western Blot: 1/500-1/2,000
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 75.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase (ALP Produits)

Synonymes: anticorps ALP, anticorps ALP1, anticorps CRASH, anticorps ECK0378, anticorps JW0374, anticorps psiA, anticorps 2410004D18Rik, anticorps AU040643, anticorps AW060375, anticorps ILC, anticorps CTACK, anticorps ESkine, anticorps Akp2, anticorps alp, anticorps zgc:56672, anticorps DDBDRAFT_0205491, anticorps DDBDRAFT_0231570, anticorps DDB_0205491, anticorps DDB_0231570, anticorps Actn2lp, anticorps Alp, anticorps asparaginase like 1, anticorps bacterial alkaline phosphatase, anticorps C-C motif chemokine ligand 27, anticorps alkaline phosphatase, liver/bone/kidney, anticorps alkaline phosphatase, anticorps PDZ and LIM domain 3, anticorps ASRGL1, anticorps phoA, anticorps Asrgl1, anticorps CCL27, anticorps alpl, anticorps alp, anticorps Pdlim3

Sujet: Synonyms: JW0374, b0383, phoA

ID gène: 945041

NCBI Accession: AP_001034

UniProt: P00634