Alkaline Phosphatase anticorps

N° du produit ABIN116596

Cet anticorps Lapin Polyclonal détecte spécifiquement Alkaline Phosphatase dans WB, IP et EIA. Il présente une réactivité avec des échantillons de E. coli.

Aperçu rapide pour Alkaline Phosphatase anticorps (ABIN116596)

Antigène: Alkaline Phosphatase (ALP)

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Alkaline Phosphatase est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-Alkaline Phosphatase anticorps

Specificité: This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.

Purification: Delipidation and Defibrination

Immunogène: Alkaline Phosphatase [E.coli].

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 μg of Alkaline Phosphatase [E. coli] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000Western Blot: 1/500-1/2,000
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 75.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase

Sujet: Synonyms: JW0374, b0383, phoA

ID gène: 945041

NCBI Accession: AP_001034

UniProt: P00634