L’anticorps anti-L-Ascorbate Oxidase Polyclonal Lapin est utilisé pour la détection de L-Ascorbate Oxidase dans des échantillons de Pumpkin. Il a été validé pour WB, EIA et IP.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: : This product has been assayed against 1.0 μg of AscorbateOxidase [cucurbita] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 6,000 to 1: 23,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent).
Concentration
85.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.