CPY anticorps

N° du produit ABIN116673

Cet anticorps anti-CPY est un anticorps Lapin Polyclonal détectant CPY dans WB, IP et EIA. Adapté pour Saccharomyces cerevisiae.

Aperçu rapide pour CPY anticorps (ABIN116673)

Antigène: CPY (Carboxypeptidase Y (CPY))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPY est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-CPY anticorps

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Carboxypeptidase Y [Baker's Yeast]. Cross reactivity against Carboxypeptidase Y from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène: Carboxypeptidase Y [Baker's Yeast].

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofCarboxypeptidase Y [Baker's Yeast] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 to 1: 3,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Détails sur CPY

Antigène: CPY (Carboxypeptidase Y (CPY))

Autre désignation: Carboxypeptidase Y

Sujet: Carboxypeptidase Y is involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.Synonyms: Carboxypeptidase YSCY, PRC1

ID gène: 855343

NCBI Accession: NP_014026

UniProt: P00729