Cet anticorps anti-Cholesterol Oxidase Polyclonal Chèvre (ABIN116685) détecte spécifiquement Cholesterol Oxidase dans WB et EIA.
L’anticorps est réactif avec des échantillons de Bactéries.
choS
Reactivité: Bactéries
ELISA
Hôte: Lapin
Polyclonal
HRP
Indications d'application
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L](Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 40,000 of thereconstitution concentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Antigène
Cholesterol Oxidase (choS)
Autre désignation
Cholesterol Oxidase
Sujet
Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.Synonyms: CHOD, Cholesterol oxidase, EC 1.1.3.6