DNASE1 anticorps

N° du produit ABIN116689

Cet anticorps Lapin Polyclonal détecte spécifiquement DNASE1 dans WB et EIA. Il présente une réactivité avec des échantillons de Boeuf (Vache).

Aperçu rapide pour DNASE1 anticorps (ABIN116689)

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNASE1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-DNASE1 anticorps

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Deoxyribonuclease I [Bovine Pancreas]. Cross reactivity against Deoxyribonuclease I from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène: Deoxyribonuclease I from bovine pancreas.

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofDeoxyribonuclease I [Bovine Pancreas] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] code R1364HRP and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 100 to 1: 1,000 of the reconstitution concentration is suggested forthis product.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Détails sur DNASE1

Antigène: DNASE1 (Deoxyribonuclease I (DNASE1))

Autre désignation: Deoxyribonuclease-1

Sujet: Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA, it was the first DNase to be crystallized, and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.Synonyms: DNASE1, DNL1, DNase I, DRNI, Deoxyribonuclease I

ID gène: 1773

NCBI Accession: NP_005214

UniProt: P24855