L’anticorps anti-Glucose Oxidase Polyclonal Lapin est utilisé pour la détection de Glucose Oxidase dans des échantillons de Aspergillus. Il a été validé pour WB, IP et EIA.
GOD
Reactivité: Others
ELISA, WB, IHC (fro), IHC (p)
Hôte: Lapin
Polyclonal
HRP
Indications d'application
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Glucose Oxidase[Aspergillus niger] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 16,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium phosphate, 0.15 M Sodium chloride, pH 7.2 containing no preservatives/stabilizers
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at -20 °C prior to opening. Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Antigène
Glucose Oxidase (GOD)
Autre désignation
Glucose Oxidase
Sujet
Glucose Oxidase is widely used for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Glucose Oxidase producing moulds such as Aspergillus and Penicillium species are used for the biological production of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.Synonyms: Beta-D-glucose:oxygen 1-oxido-reductase, Glucose oxyhydrase, gox