Cet anticorps anti-Glucose-6-Phosphate Dehydrogenase Polyclonal Chèvre (ABIN116721) détecte spécifiquement Glucose-6-Phosphate Dehydrogenase dans WB et EIA.
L’anticorps est réactif avec des échantillons de Leuconostoc.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Conjugation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofGlucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat)and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 8,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
95.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Dilute only prior to immediate use . Avoid cycles of freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at -20 °C prior to opening. This product is stable for one month at 2-8 °C as an undiluted liquid. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature.
Antigène
Glucose-6-Phosphate Dehydrogenase (G6PD)
Autre désignation
G6PD
Sujet
Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase