Lactate Dehydrogenase A anticorps

N° du produit ABIN116756

L’anticorps Chèvre Polyclonal anti-Lactate Dehydrogenase A a été validé pour WB, IP, IF et EIA. Il convient pour détecter Lactate Dehydrogenase A dans des échantillons de Lapin.

Aperçu rapide pour Lactate Dehydrogenase A anticorps (ABIN116756)

Antigène: Lactate Dehydrogenase A (LDHA)

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactate Dehydrogenase A est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-Lactate Dehydrogenase A anticorps

Séquence: Rabbit Lactate Dehydrogen ase A chain (muscle), 332 aa, predicted MW 36.6kDa 1 maalkdqlih nllkeehvpq nkitvvgvga vgmacaisil mkdladelal vdvmedklkg 61 emmdlqhgsl flrtpkivsg kdysvtansk lviitagarq qegesrlnlv qrnvnifkfi 121 ipnvvkysph ckllvvsnpv diltyvawki sgfpknrvig sgcnldsarf rylmgerlgv 181 halschgwil gehgdssvpv wsgmnvagvs lktlhpelgt dadkeqwkqv hkqvvdsaye 241 viklkgyttw aiglsvadla esimknlrrv hpistmlkgl ygikedvfls vpcvlgqngi 301 sdvvkvtlts eeeahlkksa dtlwgiqkel qf

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.

Purification: Prepared from monospecific antiserum by delipidation and defibrination

Immunogène: Full length lactate dehydrogenase protein isolated from Rabbit muscle.

Information d'application

Indications d'application: Suitable for use in ELISA (1/4,000-1/20,000) and Western blot (1/500-1/2,000). Expect aband approximately 36 kDa in size corresponding to LDH western blotting in theappropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent)

Concentration: 82 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide, None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Lactate Dehydrogenase A

Antigène: Lactate Dehydrogenase A (LDHA)

Autre désignation: LDHA

Sujet: Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.Synonyms: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, NY-REN-59, PIG19, Renal carcinoma antigen NY-REN-59

ID gène: 100009107

NCBI Accession: NP_001075746

UniProt: P13491

Pathways: L'effet Warburg