PKM2 anticorps

N° du produit ABIN116809

Cet anticorps Chèvre Polyclonal détecte spécifiquement PKM2 dans WB, IP et EIA. Il présente une réactivité avec des échantillons de Lapin.

Aperçu rapide pour PKM2 anticorps (ABIN116809)

Antigène: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-PKM2 anticorps

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Cross reactivity may occur against other mammalian Pyruvate Kinase, but were not specifically determined.

Purification: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogène: Pyruvate Kinase from Rabbit Muscle.

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation. Recommended Dilutions: This product has been assayed against 1.0 g of Pyruvate Kinase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 12,000 ofthe reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 40.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur PKM2

Antigène: PKM2 (Pyruvate Kinase M2 (PKM2))

Autre désignation: PKM2

Sujet: Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1

ID gène: 100008676

NCBI Accession: NP_001182573

UniProt: P11974

Pathways: L'effet Warburg