Recombinant alpha Tubulin anticorps

N° du produit ABIN1169084

L’anticorps Human Monoclonal anti-alpha Tubulin a été validé pour WB et ICC. Il convient pour détecter alpha Tubulin dans des échantillons de Humain, Souris et Boeuf (Vache). Il y a 2+ publications disponibles.

Aperçu rapide pour Recombinant alpha Tubulin anticorps (ABIN1169084)

Antigène: alpha Tubulin (TUBA1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Boeuf (Vache)

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: F2C

Détail du produit anti-alpha Tubulin anticorps

Specificité: Recognizes mouse, bovine and human alpha-tubulin.

Réactivité croisée: Boeuf (Vache), Humain, Souris

Purification: Purified from HEK 293 cell culture supernatant.

Pureté: >95 % (SDS-PAGE)

Immunogène: Bovine brain tubulin.

Isotype: IgG2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Références pour anticorps anti-alpha Tubulin (ABIN1169084)

Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." dans: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." dans: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: alpha-Tubulin

Sujet: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

Pathways: Dynamique des Microtubules