Hub1 anticorps

N° du produit ABIN116954

L’anticorps anti-Hub1 Polyclonal Lapin est utilisé pour la détection de Hub1 dans des échantillons de Saccharomyces cerevisiae. Il a été validé pour WB et EIA.

Aperçu rapide pour Hub1 anticorps (ABIN116954)

Antigène: Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Hub1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Hub1 anticorps

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Purification: A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Immunogène: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.

Information d'application

Indications d'application: This purified polyclonal antibody reacts with yeast Hub1 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 9.7 kDa band corresponding to yeast Hub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur Hub1

Antigène: Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))

Autre désignation: HUB1

Sujet: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Hub1 ("Homologous to UBiquitin") may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation. HUB1 has close homologs in other species including humans. The human homologs is called Ubl5.Synonyms: Ubiquitin-like modifier HUB1, YNR032C-A

ID gène: 855767

NCBI Accession: NP_014430

UniProt: Q6Q546