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PPARG anticorps (AA 149-273) (Biotin)

Cet anticorps Lapin Polyclonal détecte spécifiquement PPARG dans WB, IHC et ICC. Il présente une réactivité envers Souris.
N° du produit ABIN1173892

Aperçu rapide pour PPARG anticorps (AA 149-273) (Biotin) (ABIN1173892)

Antigène

Voir toutes PPARG Anticorps
PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivité

  • 166
  • 115
  • 102
  • 34
  • 33
  • 24
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  • 11
  • 6
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  • 2
  • 1
Souris

Hôte

  • 158
  • 25
  • 6
  • 2
Lapin

Clonalité

  • 165
  • 27
Polyclonal

Conjugué

  • 92
  • 14
  • 11
  • 6
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  • 4
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  • 2
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Cet anticorp PPARG est conjugé à/à la Biotin

Application

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  • 45
  • 29
  • 25
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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Épitope

    • 26
    • 17
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    • 12
    • 10
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    • 1
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    • 1
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    • 1
    • 1
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    • 1
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    • 1
    AA 149-273

    Fonction

    Biotin-Linked Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)

    Séquence

    MGHHHHHHSG SEF- FH YGVHACEGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY IKSFPLTKAK ARAILTGKTT DKS

    Specificité

    The antibody is a rabbit polyclonal antibody raised against PPARg. It has been selected for its ability to recognize PPARg in immunohistochemical staining and western blotting.

     Réactivité croisée

    Humain, Rat

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    Recombinant PPARg expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against PPARg conjugated to biotin.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

    Autre désignation

    Peroxisome Proliferator Activated Receptor Gamma

    Sujet

    PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3

    Pathways

    Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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