SFRP5 anticorps (AA 45-162) (Biotin)

N° du produit ABIN1175833

Détail du produit anti-SFRP5 anticorps (Biotin)

Antigène: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Épitope: AA 45-162

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SFRP5 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Séquence: MGHHHHHHSG SEF-SKPPQC LDIPADLPLC HTVGYKRMRL PNLLEHESLA EVKQQASSWL PLLAKRCHSD TQVFLCSLFA PVCLDRPIYP CRSLCEAVRA GCAPLMEAYG FPWPEMLHCH KFPLDNDLCI AV

Specificité: It has been selected for its ability to recognize SFRP5 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant SFRP5 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against SFRP5 conjugated to biotin.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant SFRP5 (Ser45~Val162) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur SFRP5

Antigène: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Autre désignation: Secreted Frizzled Related Protein 5 (SFRP5) (SFRP5 Produits)

Synonymes: anticorps SARP3, anticorps AI605071, anticorps D144, anticorps frp1, anticorps zgc:101849, anticorps Sfrp5, anticorps XsFRP5, anticorps frp5, anticorps sFRP-5, anticorps sarp3, anticorps sfrp5, anticorps sfrp5-a, anticorps sfrp5-b, anticorps secreted frizzled related protein 5, anticorps secreted frizzled-related sequence protein 5, anticorps secreted frizzled-related protein 5, anticorps secreted frizzled-related protein 5 L homeolog, anticorps secreted frizzled-related protein 5 S homeolog, anticorps SFRP5, anticorps Sfrp5, anticorps sfrp5, anticorps sfrp5.L, anticorps sfrp5.S

Pathways: Signalisation WNT