CD40 anticorps

N° du produit ABIN1176841

Cet anticorps Hamster arménien Monoclonal détecte spécifiquement CD40 dans FACS et BR. Il présente une réactivité envers Souris et Rat et a été mentionné dans 15+ publications.

Aperçu rapide pour CD40 anticorps (ABIN1176841)

Antigène: CD40

Reactivité: Souris, Rat

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: HM40-3

Détail du produit anti-CD40 anticorps

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 ng/μg of protein.

Immunogène: (BALB/c x NZB)F1 mouse-derived lymphoma WEHI-231

Isotype: IgM kappa

Information d'application

Indications d'application: For immunohistochemical staining of mouse tissue, we recommend the use of purified anti-mouse CD40 mAb 3/23 in our special formulation for immunohistochemistry, Cat. No. 550285. 553721 Note: This product may appear to contain aggregation and/or precipitation of the IgM antibody. Investigators are advised to briefly spin down any particulate matter.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4°C.

Références pour anticorps anti-CD40 (ABIN1176841)

Turner, Rakhmilevich, Burdelya, Neal, Imboden, Sondel, Yu: "Anti-CD40 antibody induces antitumor and antimetastatic effects: the role of NK cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 89-94, (2001) (PubMed).

Trinité, Voisine, Yagita, Josien: "A subset of cytolytic dendritic cells in rat." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, (2000) (PubMed).

Leifeld, Trautwein, Dumoulin, Manns, Sauerbruch, Spengler: "Enhanced expression of CD80 (B7-1), CD86 (B7-2), and CD40 and their ligands CD28 and CD154 in fulminant hepatic failure." dans: The American journal of pathology, Vol. 154, Issue 6, pp. 1711-20, (1999) (PubMed).

Akiba, Oshima, Takeda, Atsuta, Nakano, Nakajima, Nohara, Yagita, Okumura: "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7058-66, (1999) (PubMed).

Tomura, Yu, Ahn, Yamashita, Yang, Ono, Hamaoka, Kawano, Taniguchi, Koezuka, Fujiwara: "A novel function of Valpha14+CD4+NKT cells: stimulation of IL-12 production by antigen-presenting cells in the innate immune system." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 93-101, (1999) (PubMed).

Grewal, Flavell: "CD40 and CD154 in cell-mediated immunity." dans: Annual review of immunology, Vol. 16, pp. 111-35, (1998) (PubMed).

Ridge, Di Rosa, Matzinger: "A conditioned dendritic cell can be a temporal bridge between a CD4+ T-helper and a T-killer cell." dans: Nature, Vol. 393, Issue 6684, pp. 474-8, (1998) (PubMed).

Kaneko, Hirose, Abe, Yagita, Okumura, Shirai: "CD40-mediated stimulation of B1 and B2 cells: implication in autoantibody production in murine lupus." dans: European journal of immunology, Vol. 26, Issue 12, pp. 3061-5, (1997) (PubMed).

Kawano, Cui, Koezuka, Toura, Kaneko, Motoki, Ueno, Nakagawa, Sato, Kondo, Koseki, Taniguchi: "CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides." dans: Science (New York, N.Y.), Vol. 278, Issue 5343, pp. 1626-9, (1997) (PubMed).

Kashiwada, Kaneko, Yagita, Okumura, Takemori: "Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells." dans: European journal of immunology, Vol. 26, Issue 7, pp. 1451-8, (1996) (PubMed).

Inaba, Inaba, Fukuba, Mori, Haruna, Doi, Adachi, Iwai, Hosaka, Hisha: "Activation of thymic B cells by signals of CD40 molecules plus interleukin-10." dans: European journal of immunology, Vol. 25, Issue 5, pp. 1244-8, (1995) (PubMed).

Foy, Laman, Ledbetter, Aruffo, Claassen, Noelle: "gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory." dans: The Journal of experimental medicine, Vol. 180, Issue 1, pp. 157-63, (1994) (PubMed).

Inaba, Witmer-Pack, Inaba, Hathcock, Sakuta, Azuma, Yagita, Okumura, Linsley, Ikehara, Muramatsu, Hodes, Steinman: "The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro." dans: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1849-60, (1994) (PubMed).

Parry, Hasbold, Holman, Klaus: "Hypercross-linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL-4 and anti-CD40." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2821-9, (1994) (PubMed).

Noelle, Ledbetter, Aruffo: "CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation." dans: Immunology today, Vol. 13, Issue 11, pp. 431-3, (1993) (PubMed).

Détails sur CD40

Antigène: CD40

Autre désignation: CD40

Sujet: The HM40-3 antibody reacts with CD40, a 40-50-kDa glycoprotein expressed on B lymphocytes and other antigen-presenting cells. The CD40 molecule has a central role in B-cell growth and differentiation. Furthermore, interactions of CD40 with its ligand, CD154, are involved in the initiation and effector stages of cell-mediated immune responses. CD40 may be involved in the triggering of NK cells and NK-T cells. Soluble HM40-3 antibody stimulates splenic and peritoneal B cells to proliferate in vitro. This antibody also induces spleen B cells to express the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). HM40-3 mAb has been demonstrated to inhibit the binding of soluble CD154 (gp39, CD40 Ligand) to soluble CD40 and to cell-surface CD40. This hamster mAb to a mouse leukocyte antigen has been observed to cross-react with similar populations of Lewis, Sprague-Dawley, and LOU16 rat leukocytes.

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints