CD31 anticorps

N° du produit ABIN1176844

Cet anticorps Rat Monoclonal détecte spécifiquement CD31 dans FACS, IP, IHC (f), IHC (fro), IHC (zinc) et BR. Il présente une réactivité envers Souris et a été mentionné dans 9+ publications.

Aperçu rapide pour CD31 anticorps (ABIN1176844)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Blocking Reagent (BR)

Clone: MEC 13-3

Détail du produit anti-CD31 anticorps

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: 129/Sv mouse-derived endothelioma cell line tEnd.1

Isotype: IgG2a kappa

Information d'application

Indications d'application: For IHC, we recommend the use of purified MEC13.3 mAb in our special formulation for immunohistochemistry, Cat. No. 550274.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-CD31 (ABIN1176844)

Horenstein, Stockinger, Imhof, Malavasi: "CD38 binding to human myeloid cells is mediated by mouse and human CD31." dans: The Biochemical journal, Vol. 330 ( Pt 3), pp. 1129-35, (1998) (PubMed).

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." dans: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Ling, Luxenberg, Wang, Nickbarg, Leenen, Neben, Kobayashi: "Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31)." dans: European journal of immunology, Vol. 27, Issue 2, pp. 509-14, (1997) (PubMed).

Suri, Jones, Patan, Bartunkova, Maisonpierre, Davis, Sato, Yancopoulos: "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis." dans: Cell, Vol. 87, Issue 7, pp. 1171-80, (1997) (PubMed).

Vanzulli, Gazzaniga, Braidot, Vecchi, Mantovani, Wainstok de Calmanovici: "Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors." dans: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 21, Issue 1, pp. 39-46, (1997) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." dans: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." dans: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Rosenblum, Murata, Nelson, Werner, Ranken, Harmon: "Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles." dans: The American journal of pathology, Vol. 145, Issue 1, pp. 33-6, (1994) (PubMed).

Vecchi, Garlanda, Lampugnani, Resnati, Matteucci, Stoppacciaro, Schnurch, Risau, Ruco, Mantovani: "Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium." dans: European journal of cell biology, Vol. 63, Issue 2, pp. 247-54, (1994) (PubMed).

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: CD31 (PECAM-1)

Sujet: The MEC13.3 antibody reacts with CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development, this alternative splicing is involved in the regulation of ligand specificity. CD38 and vitronectin receptor (alphavbeta3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The MEC13.3 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.
Synonyms: PECAM-1

Pathways: Regulation of Actin Filament Polymerization