Integrin beta 3 anticorps

N° du produit ABIN1177004

L’anticorps Hamster arménien Monoclonal anti-Integrin beta 3 a été validé pour FACS, IHC (fro), IHC (f) et BR. Il convient pour détecter Integrin beta 3 dans des échantillons de Souris et Rat. Il y a 7+ publications disponibles.

Aperçu rapide pour Integrin beta 3 anticorps (ABIN1177004)

Antigène: Integrin beta 3 (ITGB3)

Reactivité: Souris, Rat

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp Integrin beta 3 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Blocking Reagent (BR)

Clone: 2C9-G2

Détail du produit anti-Integrin beta 3 anticorps

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Mouse T-cell Hybridoma 2B4 Vitronectin Receptor

Isotype: IgG1 kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-Integrin beta 3 (ABIN1177004)

Ashkar, Weber, Panoutsakopoulou, Sanchirico, Jansson, Zawaideh, Rittling, Denhardt, Glimcher, Cantor: "Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity." dans: Science (New York, N.Y.), Vol. 287, Issue 5454, pp. 860-4, (2000) (PubMed).

Wu, Mogford, Platts, Davis, Meininger, Davis: "Modulation of calcium current in arteriolar smooth muscle by alphav beta3 and alpha5 beta1 integrin ligands." dans: The Journal of cell biology, Vol. 143, Issue 1, pp. 241-52, (1998) (PubMed).

Frieser, Hallmann, Johansson, Vestweber, Goodman, Sorokin: "Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins." dans: European journal of immunology, Vol. 26, Issue 12, pp. 3127-36, (1997) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." dans: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

Yasuda, Hasunuma, Adachi, Sekine, Sakanishi, Hashimoto, Ra, Yagita, Okumura: "Expression and function of fibronectin binding integrins on rat mast cells." dans: International immunology, Vol. 7, Issue 2, pp. 251-8, (1995) (PubMed).

Kieffer, Phillips: "Platelet membrane glycoproteins: functions in cellular interactions." dans: Annual review of cell biology, Vol. 6, pp. 329-57, (1991) (PubMed).

Moulder, Roberts, Shevach, Coligan: "The mouse vitronectin receptor is a T cell activation antigen." dans: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 343-7, (1991) (PubMed).

Détails sur Integrin beta 3

Antigène: Integrin beta 3 (ITGB3)

Autre désignation: CD61

Sujet: The 2C9.G2 antibody reacts with the integrin beta3 chain (CD61), which associates with the integrin alphav chain (CD51) to form the vitronectin receptor and with the alphaIIb chain (CD41) to form the gpIIb/IIIa complex. Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is also mediated by the binding of alphavbeta3 integrin or vitronectin receptor to CD31 (PECAM-1). In addition, interaction of the alphavbeta3 integrin with its ligands regulates the L-type Ca2+ channel in vascular smooth muscle cells, possibly mediating vasodilatory responses to injury. Soluble and insoluble 2C9.G2 mAb mimics the effect of the natural ligands in smooth muscle cells from rat cremaster arterioles. Furthermore, osteopontin, also named Eta-1, is a cytokine that binds to alphavbeta3. CD61 is expressed on platelets, activated T lymphocytes, polymorphonuclear granulocytes, and blastocysts. Cross-reactivity of mAb 2C9.G2 to rat mast cells and platelets has been observed by flow cytometric analysis. mAb 2C9.G2 has been demonstrated to block binding of rat and mouse cells to fibronectin.
Synonyms: Integrin beta3 chain

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex