PDGFRB anticorps (pTyr857)

N° du produit ABIN1177134

Cet anticorps anti-PDGFRB est un anticorps Souris Monoclonal détectant PDGFRB dans ICS. Adapté pour Humain et Souris.

Aperçu rapide pour PDGFRB anticorps (pTyr857) (ABIN1177134)

Antigène: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDGFRB est non-conjugé

Application: Intracellular Staining (ICS)

Clone: J24-618

Détail du produit anti-PDGFRB anticorps

Épitope: pTyr857

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human PDGFRbeta Peptide

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed by gel filtration chromatography. Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Détails sur PDGFRB

Antigène: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Sujet: Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma. The J24-618 monoclonal antibody recognizes the phosphorylated tyrosine 857 (pY857) in the tyrosine kinase domain 2 of CD140b, which is required for maximal receptor kinase activity. The orthologous phosphorylation site in mouse PDGFRbeta is Y856.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling