PDGFRB anticorps (pTyr771) (PE)

N° du produit ABIN1177135

Cet anticorps Souris Monoclonal détecte spécifiquement PDGFRB dans ICS. Il présente une réactivité envers Humain et Souris et a été mentionné dans 1 publication.

Aperçu rapide pour PDGFRB anticorps (pTyr771) (PE) (ABIN1177135)

Antigène: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDGFRB est conjugé à/à la PE

Application: Intracellular Staining (ICS)

Clone: J23-1044

Détail du produit anti-PDGFRB anticorps (PE)

Épitope: pTyr771

Marque: BD Phosflow™

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography

Immunogène: Phosphorylated Human PDGFRbeta Peptide

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-PDGFRB (PE) (ABIN1177135)

Ekman, Kallin, Engström, Heldin, Rönnstrand: "SHP-2 is involved in heterodimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor." dans: Oncogene, Vol. 21, Issue 12, pp. 1870-5, (2002) (PubMed).

Détails sur PDGFRB

Antigène: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Sujet: Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma. The J23-1044 monoclonal antibody recognizes the phosphorylated tyrosine 771 (pY771) in the kinase insert domain of CD140b. pY771 interacts with GTPase-activating protein, a negative regulator of Ras, and weakly with Shc, which indirectly promotes the activation of Ras.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling