PLK1 anticorps (pThr210) (Alexa Fluor 647)

N° du produit ABIN1177152

Cité dans 1 publication. Cet anticorps anti-PLK1 Monoclonal Souris conjugué à Alexa Fluor 647 (Clone K50-483) (ABIN1177152) détecte spécifiquement PLK1 dans ICS. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour PLK1 anticorps (pThr210) (Alexa Fluor 647) (ABIN1177152)

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PLK1 est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: K50-483

Détail du produit anti-PLK1 anticorps (Alexa Fluor 647)

Épitope: pThr210

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human PLK1 Peptide

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-PLK1 (Alexa Fluor 647) (ABIN1177152)

Jang, Ma, Terada, Erikson: "Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase." dans: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44115-20, (2002) (PubMed).

Détails sur PLK1

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Autre désignation: PLK1

Sujet: Polo-like kinase (PLK1) is a serine/threonine kinase with structural similarities to Drosophila's Polo kinase and the Cdc5p of Saccharomyces cerevisiae. Like its invertebrate counterparts, PLK1 activity is required for DNA synthesis and is regulated throughout the cell cycle. Furthermore, PLK1 is highly expressed in primary tumors. It associates with the mitotic spindle during mitosis suggesting that, in addition to its role during S phase, PLK1 may play a role during chromosome segregation. This is consistent with its potential role in cancer development. Threonine 210 (T210) is one of the major phoshorylation sites in activated PLK1 obtained from human mitotic cells. The K50-483 monoclonal antibody recognizes the phosphorylated T210 of human PLK1.

Pathways: Cycle Cellulaire, M Phase