Retinoblastoma 1 anticorps (pSer780) (Alexa Fluor 488)

N° du produit ABIN1177158

Cet anticorps anti-Retinoblastoma 1 est un anticorps Souris Monoclonal détectant Retinoblastoma 1 dans ICS. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour Retinoblastoma 1 anticorps (pSer780) (Alexa Fluor 488) (ABIN1177158)

Antigène: Retinoblastoma 1 (RB1)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Retinoblastoma 1 est conjugé à/à la Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: J146-35

Détail du produit anti-Retinoblastoma 1 anticorps (Alexa Fluor 488)

Épitope: pSer780

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human Rb

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Références pour anticorps anti-Retinoblastoma 1 (Alexa Fluor 488) (ABIN1177158)

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." dans: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, (1997) (PubMed).

Détails sur Retinoblastoma 1

Antigène: Retinoblastoma 1 (RB1)

Autre désignation: Rb

Sujet: The retinoblastoma gene product (Rb) is well known as a tumor suppressor and is either absent or mutated in many human tumors. Retrovirus-mediated gene transfer of the wild-type Rb gene into several Rb mutant neoplastic cell lines suppresses their tumorgenicity. Rb is a 110-kDa nuclear phosphoprotein that undergoes differential phosphorylation during the cell cycle. During G1 phase, Rb is predominantly in a hypophosphorylated state. It becomes increasingly phosphorylated throughout the cell cycle until late mitosis, when substantial dephosphorylation occurs. Hypophosphorylated Rb interacts with a number of cellular proteins including the E2F transcription factor, several cyclins, RBP-1, RBP-2, c-Abl, c-myc, N-myc, and p46. Phosphorylation of Rb at various sites, by Cyclin-dependent protein kinases, inhibits the binding of Rb to these proteins. Rb is thought to mediate its effects, in part, via the repression of genes required for proliferation. For example, Rb is specifically recruited to promoters containing E2F sites and actively represses E2F mediated transcription. Rb also stimulates the activity of other transcription factors, although the mechanisms are less clearly defined. Thus, Rb appears to regulate transcription in its aim to control cell growth. The J146-35 monoclonal antibody recognizes Rb phosphorylated at serine 780 (pS780), which affects Rb binding to E2F. The orthologous phosphorylation sites in mouse and rat Rb are serines 773 and 751, respectively.

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy